Protein expression and purification

Identification of a novel β-N-acetylhexosaminidase (Pcb-NAHA1) from marine Zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea)

Kinetics / Sequence alignment / Anthozoa / Animals / Affinity chromatography / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Protein Expression / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology / Molecular Sequence Data / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Protein Expression / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology / Molecular Sequence Data

Download

Identification of a novel β-N-acetylhexosaminidase (Pcb-NAHA1) from marine Zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea)

Kinetics / Sequence alignment / Anthozoa / Animals / Affinity chromatography / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Protein Expression / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Protein Expression / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology

Download

Identification of a novel β-N-acetylhexosaminidase (Pcb-NAHA1) from marine Zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea)

Kinetics / Sequence alignment / Anthozoa / Animals / Affinity chromatography / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Protein Expression / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Protein Expression / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology

Download

Identification of a novel β-N-acetylhexosaminidase (Pcb-NAHA1) from marine Zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea)

Kinetics / Sequence alignment / Anthozoa / Animals / Affinity chromatography / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Protein Expression / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Protein Expression / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology

Download

Heterologous expression and physicochemical characterization of a fungal dye-decolorizing peroxidase from Auricularia auricula-judae

Kinetics / Peroxidase / Escherichia coli / Protein expression and purification / Substrate Specificity / Amino Acid Sequence / Oxidation-Reduction / Biochemistry and cell biology / BASIDIOMYCOTA / Amino Acid Sequence / Oxidation-Reduction / Biochemistry and cell biology / BASIDIOMYCOTA

Download

Purified promyelocytic leukemia coiled-coil aggregates as a tetramer displaying low α-helical content

Chromatography / Transcription Factors / Sequence Analysis / Humans / Circular Dichroism / Peptides / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Affinity chromatography / High Pressure Liquid Chromatography / Protein expression and purification / Protein Complex Detection / Secondary Structure / Protein Secondary Structure Prediction / Time Factors / Heptad Repeat / Amino Acid Profile / Glutathione Transferase / Amino Acid Sequence / Fusion Protein / Acute Promyelocytic Leukemia / Biochemistry and cell biology / Peptides / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Affinity chromatography / High Pressure Liquid Chromatography / Protein expression and purification / Protein Complex Detection / Secondary Structure / Protein Secondary Structure Prediction / Time Factors / Heptad Repeat / Amino Acid Profile / Glutathione Transferase / Amino Acid Sequence / Fusion Protein / Acute Promyelocytic Leukemia / Biochemistry and cell biology

Download

Xenopus Phospho-CDK7/Cyclin H Expressed in Baculoviral-Infected Insect Cells

Mass Spectrometry / Cell Cycle / Cell line / Animals / Threonine / Phosphorylation / Molecular cloning / Protein expression and purification / Xenopus laevis / Protein Expression / Protein Kinase / Biochemistry and cell biology / Phosphorylation / Molecular cloning / Protein expression and purification / Xenopus laevis / Protein Expression / Protein Kinase / Biochemistry and cell biology

Download

A novel chimeric MOMP antigen expressed in Escherichia coli, Arabidopsis thaliana, and Daucus carota as a potential Chlamydia trachomatis vaccine candidate

Chlamydia trachomatis / Escherichia coli / Electroporation / Animals / Genetic transformation / Arabidopsis / Solubility / Molecular cloning / Protein expression and purification / Rabbits / Amino Acid Sequence / Agrobacterium / Recombinant Proteins / Daucus Carota / Biochemistry and cell biology / Immunoblotting / Arabidopsis / Solubility / Molecular cloning / Protein expression and purification / Rabbits / Amino Acid Sequence / Agrobacterium / Recombinant Proteins / Daucus Carota / Biochemistry and cell biology / Immunoblotting

Download

Heterologous expression and physicochemical characterization of a fungal dye-decolorizing peroxidase from Auricularia auricula-judae

Kinetics / Peroxidase / Escherichia coli / Protein expression and purification / Substrate Specificity / Amino Acid Sequence / Oxidation-Reduction / Biochemistry and cell biology / BASIDIOMYCOTA / Amino Acid Sequence / Oxidation-Reduction / Biochemistry and cell biology / BASIDIOMYCOTA

Download

Strategies for bacterial expression of protein–peptide complexes: Application to solubilization of papillomavirus E6

Gene expression / Protein Structure and Function / Sequence alignment / Escherichia coli / Protein Aggregation / Peptides / Structure Analysis / Solubility / Protein expression and purification / Amino Acid Sequence / Protein Binding / Biochemistry and cell biology / Bovine papillomavirus / Peptides / Structure Analysis / Solubility / Protein expression and purification / Amino Acid Sequence / Protein Binding / Biochemistry and cell biology / Bovine papillomavirus

Download

Identification of a novel β-N-acetylhexosaminidase (Pcb-NAHA1) from marine Zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea)

Kinetics / Sequence alignment / Anthozoa / Animals / Affinity chromatography / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology / Enzyme / Protein expression and purification / Specific Activity / Substrate Specificity / Enzyme activity / Molecular Mass / Amino Acid Sequence / Elevated Temperature / Size Exclusion Chromatography / Ammonium Sulfate / Biochemistry and cell biology

Download

Purified promyelocytic leukemia coiled-coil aggregates as a tetramer displaying low α-helical content

Chromatography / Transcription Factors / Sequence Analysis / Humans / HPLC / Circular Dichroism / Peptides / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Affinity chromatography / High Pressure Liquid Chromatography / Protein expression and purification / Protein Complex Detection / Secondary Structure / Protein Secondary Structure Prediction / Time Factors / Heptad Repeat / Amino Acid Profile / Glutathione Transferase / Amino Acid Sequence / Fusion Protein / Acute Promyelocytic Leukemia / Biochemistry and cell biology / Molecular Sequence Data / Circular Dichroism / Peptides / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Affinity chromatography / High Pressure Liquid Chromatography / Protein expression and purification / Protein Complex Detection / Secondary Structure / Protein Secondary Structure Prediction / Time Factors / Heptad Repeat / Amino Acid Profile / Glutathione Transferase / Amino Acid Sequence / Fusion Protein / Acute Promyelocytic Leukemia / Biochemistry and cell biology / Molecular Sequence Data

Download